Coding

Part:BBa_K3408001:Design

Designed by: Fengzhi Yuan   Group: iGEM20_NAU-CHINA   (2020-10-03)


Phy(ycD)(Phytase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 359
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have optimized the phy(ycD) on ThermoFisher SCIENTIFIC(https://www.thermofisher.com/cn/zh/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html) to better use it in our engineered bacteria.

Source

The sequence is found through Genebank https://www.ncbi.nlm.nih.gov/nuccore/525671900

References

[1] Wang Xi, Lu Wenliang, Yao Mingze, et al. Heterologous expression and purification of the phytase phy (ycD) gene from Bacillus subtilis in Escherichia coli [J]. Applied and Environmental Biology,2014,20(02):295-299.